In Animal Ark, Mandy Hope and her friend James Hunter help helpless and injured animals throughout the series, and sometimes she helps perished animals on a "mission". Her parents, Adam and Emily Hope, are vets in Animal Ark (the veterinary practice that gives the series its name), which comes in handy in helping the animals she comes across. Mandy has three rabbits, Flopsy, Mopsy, and Cottontail. She is definite that she will become a vet and help sick animals in need. Other characters are Simon, a fellow nurse, and Jean Knox, the receptionist. There is also a black lab named "Blackie" that belongs to James who sometimes goes on adventures. Mandy finds that many people are actually very fond of animals and have a heart of gold. When she isn't working with animals, she is helping her parents with their work.
A film crew arrives in town, and Mandy offers to look after some of the animal stars. But the most important stars. But the most important animal, Charley the Labrador, suddenly disappears.
Can Mandy and her friend James find the dog before something terrible happens?